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Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter. Establishment of a permanent cell line HEL-R66 from human embryonic lung cells with high 6410 to viruses.

Controlled transcription of a human alpha-interferon gene introduced into mouse L cells. Regulation of gene expression by interferons: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Structure of a chromosomal gene for human interferon beta. High efficiency DNA-mediated transformation of primate cells. Images in this article Image on p.

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Sequencing end-labeled DNA with base-specific chemical cleavages. BK viral enhancer element and a human cellular homolog.

Interaction of positive and negative regulatory proteins in the galactose regulon of yeast. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. A rapid and sensitive assay system for bacterial gpt activity 461 transfected mammalian cells. Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines.


Affinity purification of sequence-specific DNA binding proteins. Regulation of cytokine gene expression. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. National Center for Biotechnology InformationU.

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The human beta-interferon gene enhancer is under negative control. 410 nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.

Intracytoplasmic phosphorylation sites of Tac antigen p55 are not essential irv the conformation, function, and regulation of the human interleukin 2 receptor. Delimitation and properties of DNA sequences required for the regulated expression of human iirf gene. In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is involved in the transcription of IFN-beta and possibly other genes. Short and long range activation by the SV40 enhancer.

Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. Open in a separate window.

Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements.


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Heat shock factor is regulated differently in yeast and HeLa cells. A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus. Please review our privacy policy.

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Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells. Support Center Support Center. Author information Copyright and License information Disclaimer.

IRFS (IRF) – Irfb Irfs Irfsl

Human embryonic lung cells HEL-R66 are of monkey origin. Biochemistry of interferons and their actions. This article has been cited by other articles in PMC. Human beta-interferon gene expression is regulated by an inducible enhancer element. The 5′-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription.