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Establishment of a permanent igf line HEL-R66 from human embryonic lung cells with high susceptibility to viruses. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Human beta-interferon gene expression is regulated by an inducible enhancer element. Please review our privacy policy. A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes.

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Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Regulation of cytokine gene expression. High efficiency DNA-mediated transformation of primate cells. National Center for Biotechnology InformationU.

Affinity purification of sequence-specific DNA binding proteins. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.

Images in this article Image on p. The 5′-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription. The human beta-interferon irt enhancer is under negative control. BK viral enhancer element and a human cellular homolog. Human embryonic lung cells HEL-R66 are of monkey origin.


Controlled transcription of a human alpha-interferon gene introduced into ief L cells. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells.

Interaction of positive and negative regulatory proteins in the galactose regulon of yeast. Intracytoplasmic phosphorylation sites of Tac antigen p55 are not essential for the conformation, function, and regulation of the human interleukin 2 receptor.

IRGB4610DPBF Transistor IGBT 600V 10A 77W TO220AB INFINEON (IRF)

Associated Data Supplementary Materials. Changes of chromatin conformation around mouse interferon-beta gene associated with irv of interferon synthesis. Regulation of gene expression by interferons: In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is involved in the transcription of IFN-beta and possibly other genes. Short and long range activation by the SV40 enhancer. Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines.

Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter. 4601

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Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells. Heat shock factor is regulated differently in yeast and HeLa cells.

Author information Copyright and License information Disclaimer. This article has been cited by other articles in PMC. Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor. 4601 of a chromosomal gene for human interferon beta. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus.

IRF Datasheet, PDF – Datasheet Search Engine

Support Center Support Center. Sequencing end-labeled DNA with base-specific chemical cleavages. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements. Biochemistry of interferons and their actions.